2024-04-30
The DLS-LightSheet confocal is now setup with the bright field condenser. In LASX, sign in with “machine.xlhw”. I switched the condenser to allow bright field imaging (ask me for help/instructions). This system also has multi-well presets in the navigator for automated scanning of multi-well plates and chambers.
2024-04-16
A Facilities and Other Resources page has been added to the MMC website under Software-resources. Information from this page can be used in project planning and grant applications, and it is located at: https://www.columbia-mmc.org/assets/facilities/MMC_facilities_and_other_resources.pdf
2024-03-18
STELLARIS CONFOCAL WORKSHOPS, March 26, 27, 28th. Albert Ayoub, from Leica Microsystems will be conducting Stellaris small group workshops. Bring your samples, work on getting ideal settings, tile scanning, Multi-plexing, Lightning super resolution, Tausense Lifetime imaging. Email me for the signup link: jwm2175@
2024-01-24
STELLARIS CONFOCAL SEMINAR, 4-5pm, 2024-02-23, 8th floor Black Building Albert Ayoub, Confocal Specialist from Leica Microsystems will discuss the innovative features of the Stellaris 8 microscope. Attend the event to hear about cutting edge techniques and how to improve your images and use novel technology. The seminar will be in person and on Zoom. Look for our emails. Albert will also be conducting Stellaris small group workshops in March. Details to follow.
2024-01-24
DMi Station 1. We are upgrading LASX to version 3.6.1. I I will also install another hard drive on the computer. These changes should improve performance of the system.
2024-01-23
DMi Station 1. The fluorescence lamp was adjusted to reduce the appearance of tile borders on merged (stitched) tile scans. Your acqusition settings may may need slight adjustment, but the appearance should be improved.
2023-06-30
ALEXA 790 FLUORESCENT ANTIBODIES AVAILABLE. I’ve recently purchased NIR antibodies that you can use. These image nicely on the Stellaris confocal and can allow 6 or more fluorescent channels. We have D x Mouse, and G x Rabbit, we should have D x Rabbit soon.
2023-03-27
ZEISS 710 Confocal. The system has been upgraded to Windows 10. Carl Schneider from Zeiss installed a new computer and set up all the devices. We retained the the previous computer and it is now located in the office suite of the 8th floor. It should contain your previous data drive files and can be used for data processing and image analysis.
2023-03-15
ZEISS LSM 710 CONFOCAL. I replaced the 405nm laser and the settings have now been adjusted by remote session. It is now working well in tests. If you have any issues, please contact me and I can help with settings.
2023-03-14
Room 828. The cooling system has been repaired in our main imaging room. It is now working and is significantly cooler. Please contact me if you notice issues with the cooling system..
