DMi station 1 and 2 Wide Field Microscope Information

  • Leica wide field (non-confocal) microscopes located in rooms 828 (DMi 1) 832 (DMi 2).

  • Excellent for imaging whole slides or large tissue sections containing multiple fluorescence stains.

  • Excellent for reviewing the results of your staining experiments prior to performing detailed confocal scanning.

  • Four fluorescence channels DAPI, Alexa 488, Alexa 568, Alexa 647, or equivalent fluors, along with bright field.

  • Rapid stage scanning with Leica Navigator allows fast, mutiplex, high resolution tile scans of tissue on your slides.

  • DMi station 1 has deconvolution options, post acquisition.

  • Objective lenses:

    • DMi 1: 2.5X, 5X, 10X, 20X, 40X dry 0.8NA, 100x Oil 1.4NA
      DMi 1 has low magnification options.

    • DMi 2: 10X, 20X, 20X long distance, 40X, 40X long distance, 100X oil 1.4 NA
      DMi 2 has long distance (image thru culture dish) options.

Layout of the Light Sheet system, room 828.